The study shows how the concept of biodiversity mainstreaming has become a cornerstone of global biodiversity governance. It offers an overview of various entry points for mainstreaming biodiversity considerations into different thematic areas, such as in finance, policy planning cycles and monitoring frameworks, and discusses mainstreaming approaches in key sectors, i.e. land-use planning, forestry, infrastructure, and tourism. Case studies from Indonesia, Mexico and Thailand illustrate how biodiversity mainstreaming can be put into practice through national policy instruments and planning processes.